Exciting new research from Northwestern Medicine reveals that mitochondria, often referred to as the cellular ‘powerhouses’, play a crucial role in regulating the development of lung epithelial cells. These cells are responsible for the exchange of oxygen and carbon dioxide, making them essential for avoiding respiratory failure. The findings, published in Nature, shed light on the importance of mitochondrial signaling in determining cell fate.
“Over the past 20 years, we have argued that mitochondria do more than just produce ATP; they control cell state and function by generating signals, including metabolites, to control physiology. If these signals are disturbed, disease can result. This study is a compelling demonstration of that principle and highlights the role of mitochondrial signaling in determining cell fate,” said Navdeep Chandel, Ph.D., the David W. Cugell, MD, Professor of Medicine in the Division of Pulmonary and Critical Care and of Biochemistry and Molecular Genetics and senior author of the study.
SeungHye Han, MD, MPH, assistant professor of Medicine in the Division of Pulmonary and Critical Care, was lead author of the study.
Mitochondria are widely known as cellular ‘powerhouses’ for their role in producing ATP, the source of energy for cells, and it had been previously thought that mitochondrial malfunction causes disease solely due to decreased energy production for cells.
Chandel and Han’s most recent findings, however, illustrate that mitochondria have functions beyond ATP production, including controlling the cellular fate of alveolar epithelial type 2 cells, which serve partially as stem cells and also regulate gas exchange in the lungs.
In the current study, the investigators used genetic knockout techniques to delete a mitochondrial electron transport chain complex I subunit, called Ndufs2, in lung epithelial cells in gestational mice. Next, using single-cell RNA sequencing, the team observed that mitochondria involve an integrated stress response (ISR), a cellular function that’s activated to cope with metabolic stress or proteotoxicity, or the accumulation of misfolded proteins.
When mitochondria malfunctioned, the ISR increased activation and inhibited the differentiation of alveolar epithelial cells in post-natal mice, ultimately leading to respiratory failure in the mice. Interestingly, the team also noted that the cells without functional mitochondria did not die, but instead remained in a state of stalled cell differentiation, which is commonly observed in a variety of lung diseases.
“We’re now examining the possibility that mitochondrial ISR signaling could be disrupted during lung repair after injury resulting in lung diseases like pulmonary fibrosis or prolonged viral pneumonia. If this is true, it could open new avenues for treatments that target mitochondria-dependent ISR signaling in diseases involving lung damage and repair,” Han said.
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